The Core Unit Signalling Technologies

The core unit provides assays based on the AlphaScreen® proximity assay technology (1). The modular nature of this assay system allows its application to a large number of signalling components in medium- and high-throughput formats. The available commercial kits include systems for the quantification of second messenger molecules, protein-protein or DNA-protein interactions and kinase activity. In addition, customized assays for medium and high affinity interactions can be readily established. Other available methods include luminescence and fluorescence measurements as well as ligand binding assays.

Furthermore, a label-free detection platform that uses optical biosensors to measure cellular signal transduction and other biochemical reactions like ligand and substrate binding is available.  The  high sensitivity of the optical biosensor technology allows it to measure biologically relevant cells (e.g. human primary cells, endogenously expressed cells, stem cells, etc.) as well as stable and transient overexpressing cells and cell free systems (2).

cAMP service

The core unit measures cAMP routinely by AlphaScreen technology. Each customer can use the permanently available kits, optiplates (384-well), Fusion-alpha FP plate reader and protocols including measurements with adberent and suspended cells. It is possible to measure less than 384 assay points because costumers are billed per well number. For more information and prices please contact Thomas Hermsdorf, Tel. 0341/ 97-22168.

EPIC service

Each customer can use the Corning EPIC Cell Assay Microplates (384-well) and EPIC system for lable-free measurements. The Core Unit supports protocols, all accessories and supervising for cell-based assays. It is possible to use less than 384 assay points because customers are billed per well number. For more information and prices please contact Thomas Hermsdorf, Tel. 0341/ 97-22168.

(1) www.perkinelmer.com/Catalog/Category/ID/AlphaScreen%20Assays%20and%20Reagents
(2) www.corning.com/lifesciences/epic/en/products/epic_system.aspx